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Assessment of biological condition is regarded as the effective way of evaluating cumulative impacts from nonpoint sources, which might involve habitat degradation, chemical contamination, or water withdrawal Karr 1991. Biological assessment techniques can improve evaluations of nonpoint source pollution controls or perhaps the combined effectiveness of current point and nonpoint source controls by comparing biological indicators before implementation of controls. Likewise, biological attributes can be utilized to measure site-specific ecosystem reaction to remediation or mitigation activities directed at reducing nonpoint source pollution impacts or reaction to pollution prevention activities. 2.3.3 Watershed Protection Approach Since 1991, USEPA has become promoting the Watershed Protection Approach WPA like a framework for meeting the Nations remaining water resource challenges USEPA 1994c. USEPAs Office of Water has steps to reorient and coordinate point source, nonpoint source, surface waters, wetlands, coastal, ground water, and normal water programs in support with the watershed approach. USEPA also has promoted multi-organizational, multi-objective watershed management projects across the Nation. The watershed approach can be an integrated, inclusive strategy for better protecting and managing surface water and ground water resources arid achieving broader environmental protection objectives while using the naturally defined hydrologic unit the watershed because the integrating management unit. Thus, for any given watershed, the approach encompasses not just the water resource, such to be a stream, river, lake, estuary, or aquifer, but the many land that water drains for the resource. The watershed approach places focus on all issues with water resource qualityphysical, temperature, flow, mixing, habitat; chemical, conventional and toxic pollutants for instance nutrients and pesticides; and biological, health insurance integrity of biotic communities, biodiversity. As states develop their Watershed Protection Approach WPA, biological assessment and monitoring offer a way of conducting comprehensive evaluations of ecological status and improvements from restoration/rehabilitation activities. Biological assessment integrates the condition from the watershed from tributaries to mainstem from the exposure/response of indigenous aquatic communities. 2.3.4 CWA Section 303dThe TMDL Process The technical backbone from the WPA would be the TMDL process. A total maximum daily load TMDL is often a tool accustomed to achieve applicable water quality standards. The TMDL process quantifies the loading capacity of any waterbody to get a given stressor and ultimately supplies a quantitative scheme for allocating loadings or external inputs among pollutant sources USEPA 1994a. In doing so, the TMDL quantifies the relationships among sources, stressors, recommended controls, and water quality conditions. For example, a TMDL might mathematically show what sort of specified percent reduction of the pollutant is critical to reach the pollutant concentration reflected in the water quality standard. 2-6 Chapter 2: Application of Rapid Bioassessment Protocols RBPs Section 303d in the CWA requires each state to find out, according to its priority rankings, the complete maximum daily load for every waterbody or reach identified by their state as failing in order to meet, you aren't expected to satisfy, water quality standards after imposition of technology-based controls. In addition, TMDLs are crucial elements of an growing volume of state programs. For example, as increasing numbers of permits incorporate water quality-based effluent limits, TMDLs are getting to be an increasingly important component from the point-source control program. TMDLs are suitable for nonchemical together with chemical stressors USEPA 1994a. These include all stressors that contribute towards the failure in order to meet water quality standards, along with any stressor that presently threatens but isn't going to yet impair water quality. TMDLs are applicable to waterbodies afflicted with both point and nonpoint sources. Some stressors, including sediment deposition or physical improvements on instream habitat, might not exactly clearly fit traditional concepts linked to chemical stressors and loadings. For these nonchemical stressors, it will sometimes be tough to develop TMDLs as a consequence of limitations from the data or inside technical strategies to analysis and modeling. In the case of nonpoint source TMDLs, another difficulty arises as the CWA won't provide well-defined support for regulatory control actions mainly because it does for point source controls, and controls depending on another statutory authority may be necessary. Biological assessments and criteria address the cumulative impacts of most stressors, especially habitat degradation, and chemical contamination, which result within a loss of biological diversity. Biological information can assist provide an ecologically based assessment in the status of any waterbody and thus can be accustomed to decide which waterbodies need TMDLs USEPA 1997c and aid inside the ranking process by targeting waters for TMDL development which has a more accurate link between bioassessment and ecological integrity. Finally, the TMDL process is usually a geographically-based method of preparing load and wasteload allocations for types of stress that will impact waterbody integrity. The geographic nature with this process is going to be complemented and enhanced if ecological regionalization is applied as portion of the bioassessment activities. Specifically, similarities among ecosystems is usually grouped into homogeneous classes of streams and rivers that gives a geographic framework for further efficient aquatic resource management. 2.3.5 CWA Section 402NPDES Permits and Individual Control Strategies All point causes of wastewater must get yourself a National Pollutant Discharge Elimination System NPDES permit or state equivalent, which regulates the facilitys launch of pollutants. The strategy to controlling and eliminating water pollution is dependant on the pollutants determined to be damaging to receiving waters additionally, on the types of such pollutants. Authority for issuing NPDES permits is established under Section 402 with the CWA USEPA 1989. Point sources are usually divided into two typesindustrial and municipal. Nationwide, around 50, 000 industrial sources, such as commercial and manufacturing facilities. Municipal sources, generally known as publicly owned treatment works POTWs, number about 15, 700 nationwide. Wastewater from municipal sources comes from domestic wastewater discharged to POTWs, as well as being the indirect turmoil industrial wastes to sewers. In addition, stormwater might be discrete or diffuse, but is additionally covered by NPDES permitting regulations. USEPA won't recommend the utilization of biological survey data since the basis for deriving an effluent limit to have an NPDES permit USEPA 1994d. Unlike chemical-specific water quality analyses, biological data usually do not measure the concentrations or degrees of chemical stressors. Instead, they directly look at the impacts of the and all stressors within the resident aquatic biota. Where Rapid Bioassessment Protocols for Use in Streams and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish, Second Edition 2-7 appropriate, biological assessment is usually used inside NPDES process USEPA 1994d to get information for the status of the waterbody where point sources may result in, or bring about, a water quality problem. In conjunction with chemical water quality and whole-effluent toxiciry data, biological data can be accustomed to detect previously unmeasured chemical water quality problems and also to evaluate the potency of implemented controls. Some states already have demonstrated the usefulness of biological data to point out the need for additional or maybe more stringent permit limits, sole-source discharge to a stream its keep is no significant nonpoint source discharge, habitat degradation, or atmospheric deposition USEPA 1994d. In these situations, the biological findings triggered additional investigations to find out the cause-and-effect relationship and also to determine the suitable limits. In this manner, biological data support regulatory evaluations and selection. Biological data can be useful in monitoring highly variable or diffuse types of pollution that happen to be treated as point sources for instance wet-weather discharges and stormwater runoff USEPA 1994d. Traditional chemical water quality monitoring is frequently only minimally informative of these types of point source pollution, along with a biological survey with their impact may be critical to effectively evaluate these discharges and associated treatment measures. 2.3.6 Ecological Risk Assessment Risk assessment is really a scientific method that includes stressor identification, receptor characterization and endpoint selection, stress-response assessment, and risk characterization USEPA 1992, Suter et al. 1993. Risk management is really a decision-making procedure that involves all of the human-health insurance ecological assessment results, considered with political, legal, economic, and ethical values, to ; develop and enforce environmental standards, criteria, and regulations Maughan 1993. Risk assessment could be performed by using an on-site basis or is usually geographically-based, watershed or regional scale, and it might be used to gauge human health threats or to distinguish ecological impairments. In early 1997, an investigation prepared using a Presidential/Congressional Commission on risk enlarged the context of risk to feature ecological in addition to public health threats Karr and Chu 1997. Biological monitoring will be the essential first step toward ecological risk assessment because doing so measures present biological conditions not merely chemical contamination and offers the methods to compare them using the conditions expected from the absence of humans Karr and Chu 1997. Results of regional bioassessment studies is usually used in watershed ecological risk assessments to cultivate broad scale geographic empirical styles of biological responses to stressors. Such models will then be used, along with exposure information, to predict risk caused by stressors in order to alternative management actions. Risks to biological resources are characterized, and causes of stress could be prioritized. Watershed risk managers can and may use such outcomes for critical management decisions. 2.3.7 USEPA Water Quality Criteria and Standards The water quality standards program, as envisioned in Section 303c in the Clean Water Act, is really a joint effort between states and USEPA. The states have primary responsibility for setting, reviewing, revising, and enforcing water quality standards. USEPA develops regulations, policies, and guidance that can help states implement this system and oversees states activities to make certain their adopted standards are consistent while using requirements with the CWA and relevant water quality standards regulations 40 CFR Part 131. USEPA has authority to examine and approve or disapprove state standards and, where necessary, to promulgate federal water quality standards. A water quality standard defines the goals of any waterbody, or even a portion thereof, by designating the utilization or uses to become made on the water, setting criteria needed to protect those uses, and preventing 2-8 Chapter 2: Application of Rapid Bioassessment Protocols RBPs degradation of water quality through antidegradation provisions. States adopt water quality standards to defend public health or welfare, increase the quality of water, and protect biological integrity. Chemical, physical, or biological stressors impact the biological characteristics of the aquatic ecosystem Gibson et al. 1996. For example, chemical stressors may lead to impaired functioning or loss of an sensitive species as well as a change in community structure. Ultimately, the quantity and intensity of stressors in the ecosystem is going to be evidenced by the change within the condition and function from the biotic community. The interactions among chemical, physical, and biological stressors along with their cumulative impacts emphasize the requirement to directly detect and look at the biota as indicators of actual water resource impairments. Sections 303 and 304 in the CWA require states to guard biological integrity as part with their water quality standards. This may be accomplished, simply, over the development and employ of biological criteria. As part of your state or tribal water quality standards program, biological criteria provides scientifically sound and detailed descriptions with the designated aquatic life use for any specific waterbody or segment. They fulfill an essential assessment function in water quality-based programs by establishing the biological benchmarks for 1 directly measuring the condition from the aquatic biota, 2 determining water quality goals and setting priorities, and 3 evaluating great and bad implemented controls and management actions. Biological criteria for aquatic systems produce an evaluation benchmark for direct assessment in the condition in the biota that reside either part or all of the lives in aquatic systems Gibson et al. 1996 by describing in narrative or numeric criteria the expected biological condition of your minimally impaired aquatic community USEPA 1990b. They can be accustomed to define ecosystem rehabilitation goals and assessment endpoints. Biological criteria supplement traditional measurements as an example, as backup for hard-to-detect chemical problems and are going to be particularly beneficial in assessing impairment as a result of nonpoint source pollution and nonchemical, physical and biological stressors. Thus, biological criteria fulfill a function missing from USEPAs traditionally chemical- oriented method of pollution control and abatement USEPA 1994d. Biologicalcriteria can also be accustomed to refine the aquatic life use classifications for the state. Each state develops a unique designated use classification system determined by the generic uses cited inside CWA, including protection and propagation of fish, shellfish, and wildlife. States frequently develop subcategories to refine and clarify designated use classes when several surface waters with distinct characteristics fit in the same use class or when waters will not fit well into any single category. As data are collected from biosurveys to formulate a biological criteria program, analysis may reveal unique and consistent differences between aquatic communities that inhabit different waters while using same designated use. Therefore, measurable biological attributes can be employed to refine aquatic life use as well as to separate 1 class of aquatic life into 2 or maybe more subclasses. For example, Ohio has generated an exceptional warmwater use class to incorporate all unique waters, not representative of regional streams and different off their standard warmwater class. Rapid Bioassessment Protocols for Use in Streams and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish, Second Edition 2-9 ELEMENTS OF BIOMONITORING 3.1 BIOSURVEYS, BIOASSAYS, AND CHEMICAL MONITORING The water quality-based strategy to pollution assessment requires various forms of data. Biosurvey techniques, such because the Rapid Bioassessment Protocols RBPs, would be better used for detecting aquatic life impairments and assessing their relative severity. Once an impairment is detected, however, additional ecological data, for example chemical and biological toxicity exams are helpful to name the causative agent, its source, and implement appropriate mitigation USEPA 1991c. Integrating information from all of these data types along with from habitat assessments, hydrological investigations, and familiarity with land use is beneficial to provide an intensive diagnostic assessment of impacts from your 5 principal factors see Karr et al. 1986, Karr 1991, Gibson et al. 1996 for description of water quality, habitat structure, power source, flow regime, and biotic interaction factors. Following mitigation, biosurveys are crucial for evaluating the strength of such control measures. Biosurveys might be used inside a planning and management framework to prioritize water quality problems for further stringent assessments and document environmental recovery following control action and rehabilitation activities. Some in the advantages of using biosurveys just for this type of monitoring are: Biological communities reflect overall ecological integrity, chemical, physical, and biological integrity. Therefore, biosurvey results directly study the status of the waterbody relative for the primary goal with the Clean Water Act CWA. Biological communities integrate the impact of different stressors and thus offer a broad measure of the aggregate impact. Communities integrate the stresses as time passes and offer an ecological measure of fluctuating environmental conditions. : Routine monitoring of biological communities could be relatively inexpensive, in particular when compared towards the cost of assessing toxic pollutants, either chemically or with toxicity tests Ohio EPA 1987. ! The status of biological communities is of direct interest towards the public as being a measure of an pollution free environment. Where criteria for specific ambient impacts don't exist, nonpoint-source impacts that degrade habitat, biological communities might be the only practical method of evaluation. Biosurvey methods possess a long-standing good use for pre and post monitoring. However, the intermediate procedures in pollution control, , identifying causes and limiting sources, require integrating information of assorted typeschemical, physical, toxicological, and/or biosurvey data. These data are important to: Rapid Bioassessment Protocols for Use in Streams and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish, Second Edition 3-1 Identify the precise stress agents causing impact: This could possibly be a easy task; but, because of the array of potentially important pollutants as well as their possible combinations, it is likely being both difficult and dear. In situations where specific chemical stress agents may be poorly understood in order to varied to gauge individually, toxicity tests can be utilized to focus specific chemical investigations or characterize generic stress agents, whole effluent or ambient toxicity. For situations where habitat degradation is prevalent, a combined biosurvey and physical habitat assessment is most successful Barbour and Stribling 1991. Identify and limit the specific types of these agents: Although biosurveys might be used to assist locate the likely origins of impact, chemical analyses and/or toxicity tests are useful to confirm the purpose sources and develop appropriate discharge limits. Impacts because of factors in addition to chemical contamination will need different ecological data. Design appropriate treatment to meet up with the prescribed limits and monitor compliance: Treatment facilities are made to remove identified chemical constituents that has a specific efficiency. Chemical data are thus required to judge treatment effectiveness. To some degree, a biological endpoint caused by toxicity testing can also be utilized to evaluate the potency of prototype treatment schemes and will seive being a design parameter. In most cases, similar parameters are limited in discharge permits and, after controls will be in place, are accustomed to monitor for compliance. Where discharges usually are not controlled by using a permit system, nonpoint-source runoff, combined sewer outfalls, and dams compliance need to be assessed when it comes to ambient standards. Improvement on the ecosystem both from restoration or rehabilitation activities might be best monitored by biosurvey techniques. Effective implementation on the water quality-based approach mandates that various monitoring techniques be considered in a larger context of water resource management. Both biological and chemical methods play critical roles inside a successful pollution control program. They should be looked at complementary instead of mutually exclusive approaches that can enhance overall program effectiveness when used appropriately. 3.2 USE OF DIFFERENT ASSEMBLAGES IN BIOSUEtVEYS The techniques presented on this document target the evaluation of water quality physicochemical constituents, habitat parameters, and analysis with the periphyton, benthic macroinvertebrate, and fish assemblages. Many State water quality agencies employ trained and experienced benthic biologists, have accumulated considerable background data on macroinvertebrates, and consider benthic surveys a good assessment tool. However, water quality standards, legislative mandate, and public opinion tend to be more directly related for the status of the waterbody being a fishery resource. For this reason, separate protocols were developed for fish and were incorporated as Chapter 8 in this particular document. The fish survey protocol relies largely on Karrs Index of Biotic Integrity IBI Kaior 1981, Karr et al. 1986, Miller et al. 1988, which uses the structure from the fish assemblage to judge water quality. The integration of functional and structural/compositional metrics, which forms the cause for the IBI, is often a common element on the rapid bioassessment approaches. The periphyton assemblage primarily algae is usually useful for water quality monitoring, but is not incorporated widely in monitoring programs. They represent the principle producer trophic level, exhibit a new range of sensitivities, and may often indicate effects only indirectly observed inside the benthic and fish communities. As inside benthic macroinvertebrate and fish assemblages, integration of structural/compositional and functional characteristics provides best way of assessing impairment Rodgers et al. 1979. 3-2 Chapter 3: Elements ofBiomonitoring In picking out the aquatic assemblage appropriate to get a particular biomonitoring situation, the great things about using each assemblage need to be considered along together with the objectives with the program. Some on the advantages of using periphyton, benthic macroinvertebrates, and fish within a biomonitoring program are presented in this particular section. References because of this list are Cairns and Dickson 1971, American Public Health Association et al. 1971, Patrick 1973, Rodgers et al. 1979, Weitzel 1979, Karr 1981, USEPA 1983, Hughes et al. 1982, and Plafkin et al. 1989. 3.2.1 Advantages of Using Periphyton Algae generally rapid reproduction rates and also short life cycles, driving them to valuable indicators of short-term impacts. As primary producers, algae are most directly impacted by physical and chemical factors. Sampling is straightforward, inexpensive, requires not enough people, and helps to create minimal impact to resident biota. Relatively standard methods are available for evaluation of functional and non-taxonomic structural biomass, chlorophyll measurements characteristics of algal communities. Algal assemblages are understanding of some pollutants which could not visibly affect other aquatic assemblages., or might only affect other organisms at higher concentrations, herbicides. 3.2.2 Advantages of Using Benthic Macroinvertebrates Macroinvertebrate assemblages are great indicators of localized conditions. Because many benthic macroinvertebrates have limited migration patterns or possibly a sessile mode of life, these are particularly well-suited for assessing site-specific impacts upstream- downstream studies. Macroinvertebrates integrate the consequences of short-term environmental variations. Most species use a complex life cycle of approximately one year or maybe more. Sensitive life stages will respond quickly to push; the entire community will respond slower. Degraded conditions is often detected by a professional biologist with a cursory examination with the benthic macroinvertebrate assemblage. Macro- invertebrates are not too difficult to identify to family; many intolerant taxa is usually identified to lessen taxonomic levels effortlessly. Benthic macroinvertebrate assemblages include species that constitute a broad array of trophic levels and pollution tolerances, thus providing strong information for interpreting cumulative effects. Sampling is relatively simple, requires not enough people and inexpensive gear, and contains minimal detrimental effect within the resident biota. Rapid Bioassessment Protocols for Use in Streams and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish, Second Edition 3-3 Benthic macroinvertebrates serve like a primary eating place for fish, including many recreationally and commercially important species. Benthic macroinvertebrates are loaded in most streams. Many small streams 1st and 2nd order, which naturally support an easy macroinvertebrate fauna, only support a fixed fish fauna. Most state water quality agencies that routinely collect biosurvey data give attention to macroinvertebrates Southerland and Stribling 1995. Many states currently have background macroinvertebrate data. Most state water quality agencies have an overabundance of expertise with invertebrates than fish. 3.2.3 Advantages of Using Fish Fish are great indicators of long-term a very extensive period effects and broad habitat conditions because they may be relatively long-lived and mobile Karr et al. 1986. Fish assemblages generally include a choice of species that represent various trophic levels omnivores, herbivores, insectivores, planktivores, piscivores. They tend to integrate results of lower trophic levels; thus, fish assemblage structure is reflective of integrated environmental health. Fish are on the top in the aquatic food web and are also consumed by humans, driving them to important for assessing contamination. Fish are easy to collect and identify for the species level. Most specimens may be sorted and identified from the field by experienced fisheries professionals, and subsequently released unharmed. Environmental requirements of the majority of fish are comparatively popular. Life history data is extensive for several species, and home elevators fish distributions is often available. Aquatic life uses water quality standards are generally characterized when it comes to fisheries coldwater, coolwater, warmwater, sport, forage. Monitoring fish provides direct evaluation of fishability and fish propagation, which emphasizes the benefit offish to anglers and commercial fishermen. Fish are the cause of nearly half in the endangered vertebrate species and subspecies hi the United States Warren and Burr 1994. 3.3 IMPORTANCE OF HABITAT ASSESSMENT The procedure for assessing physical habitat quality presented in this particular document Chapter 5 can be an integral component in the final evaluation of impairment. The matrix used to gauge habitat quality is depending on key physical characteristics on the waterbody and surrounding land, in particular the catchment from the site under investigation. All in the habitat parameters evaluated matched to overall aquatic life use and therefore are a potential method of obtaining limitation for the aquatic biota. 3-4 Chapter 3: Elements ofBiomonitoring The alteration from the physical structure from the habitat is one kind of 5 major factors from human activities explained by Karr Karr et al. 1986, Karr 1991 that degrade aquatic resources. Habitat, as structured by instream and surrounding topographical features, is often a major determinant of aquatic community potential Southwood 1977, Plafkin et al. 1989, and Barbour and Stribling 1991. Both the quality and quantity of obtainable habitat change the structure and composition of resident biological communities. Effects of such features on biological assessment results is usually minimized by sampling similar habitats in any respect stations being compared. However, when all stations aren't physically comparable, habitat characterization is particularly vital for proper interpretation of biosurvey results. Where physical habitat quality for a test site is much like that of any reference, detected impacts might be attributed to water quality factors, chemical contamination or another stressors. However, where habitat quality differs substantially from reference conditions, the question of appropriate aquatic life use designation and physical habitat alteration/restoration should be addressed. Final conclusions concerning the presence and amount of biological impairment should thus have an evaluation of habitat quality to look for the extent that habitat can be a limiting factor. The habitat characterization matrix included inside Rapid Bioassessment Protocols offers effective ways of evaluating and documenting habitat quality at each and every biosurvey station. 3.4 THE REGIONAL REFERENCE CONCEPT The issue of reference conditions is critical to your interpretation of biological surveys. Barbour et al. 1996a describe 2 varieties of reference conditions which can be currently employed in biological surveys: site- specific and regional reference. The former typically contains measurements of conditions upstream of any point source discharge or from your paired watershed. Regional reference conditions, about the other hand, include measurements coming from a population of relatively unimpaired sites in just a relatively homogeneous region and habitat type, and therefore are certainly not site-specific. The reference condition establishes the cause of making comparisons as well as for detecting use impairment; it needs to be applicable in an individual waterbody, such being a stream segment, but in addition to similar waterbodies using a regional scale Gibson et al. 1996. Although both site-specific and ecoregional references represent conditions with no influence of your particular discharge, the 2 sorts of references might not yield equivalent measurements Barbour et al. 1996a. While site-specific reference conditions represented with the upstream, downstream, or paired-site approach are desirable, they may be limited of their usefulness. Hughes 1995 shows three complications with site-specific reference conditions: 1 since they typically lack any broad study design, site-specific reference conditions possess limited convenience of extrapolation they have got only site-specific value; 2 usually site-specific reference conditions allow limited variance estimates; you will discover too few sites for robust variance evaluations because each site of interest is typically represented by one-to-three reference sites; the end result could be an incorrect assessment should the upstream site has especially good or especially poor habitat or chemical quality; and 3 they require a substantial assessment effort when considered over a statewide basis. The great things about measuring upstream reference conditions are these: 1 if carefully selected, the habitat quality is often a lot like that measured downstream of any discharge, thereby reducing complications in interpretation due to habitat differences, and a couple of impairments as a result of upstream influences using point and nonpoint sources happen to be factored in the reference condition Barbour et al. 1996a. New York DEC found that an upstream-downstream approach helps with Rapid Bioassessment Protocols for Use in Streams and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish, Second Edition 3-5 diagnosing cause-and-effect to specific discharges and increase precision Bode and Novak 1995. Where feasible, effects needs to be bracketed by establishing a set or network of sampling stations at points of accelerating distance in the impact sources. These stations will supply a cause of delineating impact and recovery zones. In significantly altered systems, channelized or heavily urbanized streams, suitable reference sites are often not available Gibson et al. 1996. In these cases, historical data or simple ecological models can be necessary to ascertain reference conditions. See Gibson et al. 1996 for much more detail. Innate regional differences happens to forests, lands with higher agricultural potential, wetlands, and waterbodies. These regional differences have already been mapped by Bailey 1976, Department of Agriculture USDA Soil Conservation Service 1981, Energy, Mines and Resources Canada 1986, and Omernik 1987. Waterbodies reflect the lands they drain Omernik 1987, Hunsaker and Levine 1995 in fact it is assumed that similar lands should produce similar waterbodies. This ecoregional approach provides robust and ecologically-meaningful regional maps that are according to an examination of countless mapped land variables. For example, hydrologic unit maps are helpful for mapping drainage patterns, but have limited value for explaining the substantial changes that appear in water quality and biota separate from stream size and river basin. Omernik 1987 provided an ecoregional framework for interpreting spatial patterns in state and national data. The geographical framework is depending on regional patterns in land-surface form, soil, potential natural vegetation, and land use, which vary across the nation. Geographic patterns of similarity among ecosystems may be grouped into ecoregions or subecoregions. Naturally occurring biotic assemblages, as components with the ecosystem, would be likely to differ among ecoregions but be relatively similar in a given ecoregion. The ecoregion concept thus supplies a geographic framework for efficient treatments for aquatic ecosystems along with their components Hughes 1985, Hughes et al. 1986, and Hughes and Larsen 1988. For example, studies in Ohio Larsen et al. 1986, Arkansas Rohm et al. 1987, and Oregon Hughes et al. 1987, Whittier et al. 1988 demonstrate that distributional patterns offish communities approximate ecoregional boundaries as defined a priori by Omernik 1987. This, subsequently, ensures that similar water quality standards, criteria, and monitoring strategies are likely for being valid within a given ecoregion, but really should be tailored to allow for the innate differences among ecoregions Ohio EPA 1987. However, some programs, for instance EMAP Klemm and Lazorchak 1994 along with the Maryland Biological Stream Survey MBSS Volstad et al. 1995 are finding that a surrogate measure of stream size catchment size is beneficial in partitioning the variability of stream segments for assessment. Hydrologic regime normally include flow regulation, water withdrawal, and whether a stream may be known as intermittent or perennial. Elevation is found to get an important classification variable when while using the benthic macroinvertebrate assemblage Barbour et al. 1992, Barbour et al. 1994, Spindler 1996. In addition, descriptors at the smaller scale can be needed to characterize streams within regions or classes. For example, while a given stream segment is classified inside of a subecoregion or other form of stream class, it might be wooded deciduous or coniferous or open in a perennial or intermittent flow regime, and represent one of numerous orders of stream size. Individual descriptors won't apply to any or all regional reference streams, nor will all conditions, deciduous, coniferous, open be present in all streams. Those streams or stream segments that represent characteristics atypical for the particular ecoregion ought to be excluded on the regional aggregate of websites and treated being a special situation. For example, Ohio EPA 1987 considered aquatic systems with unique, unusual to the ecoregion natural characteristics to become a separate aquatic life use designation exceptional warmwater aquatic life use with a statewide basis. 3-6 Chapter 3: Elements ofBiomonitoring Although the last rapid bioassessment guidance ought to be generally applicable to all or any regions with the United States, each agency will need to look at the generic criteria suggested within this document for inclusion into specific programs. To this end, the application from the regional reference concept versus the site-specific control approach will need to become examined. When Rapid Bioassessment Protocols RBPs are used to gauge impact sources upstream-downstream studies, regional reference criteria would possibly not be as vital if an unimpacted site-specific control station is usually sampled. However, every time a synoptic snapshot or trend monitoring survey is being conducted in the watershed or river basin, using regional criteria could possibly be the only methods of discerning use impairment or assessing impact. Additional investigation are going to be needed to: delineate areas classes of streamsthat differ significantly hi then: innate biological potential; locate reference sites within each stream class that fully support aquatic life uses; develop biological criteria, define optimal values for that metrics using data generated from each with the assemblages. 3.5 STATION SITING Site selection for assessment and monitoring may either be targeted, , strongly related special studies that target potential problems, or probabilistic, which offers information on the overall status or condition on the watershed, basin, or region. In a probabilistic or random sampling regime, stream characteristics can be highly dissimilar on the list of sites, but will give a more accurate assessment of biological condition during the entire area over a targeted design. Selecting sites randomly has an unbiased assessment with the condition from the waterbody at the scale higher than the individual site or stream. Thus, a company can address questions at multiple scales. Studies for 305b status and trends assessments would be best done having a probabilistic design. Most studies conducted by state water quality agencies for identification of problems and sensitive waters are done which has a targeted design. In this case, sampling sites are selected dependant on known existing problems, familiarity with upcoming events that can adversely change the waterbody such as being a development or deforestation; or installing of BMPs or habitat restoration which can be intended to improve waterbody quality. This method provides assessments of human sites or stream reaches. Studies for aquatic life use determination and the ones related to TMDLs could be done having a random watershed or older level or targeted site-specific design. To meaningfully evaluate biological condition in a very targeted design, sampling locations have to be similar enough to own similar biological expectations, which, consequently, offers a basis for comparison of impairment. If the goal connected with an assessment is to measure the effects of water chemistry degradation, comparable physical habitat really should be sampled whatsoever stations, otherwise, the differences from the biology due to a degraded habitat will be tricky to separate from those presented by chemical pollution water quality degradation. Availability of appropriate habitat at every sampling location might be established during preliminary reconnaissance. In evaluations where several stations over a waterbody are going to be compared, the station using the greatest habitat constraints with regards to productive habitat availability must be noted. The station while using least variety of productive habitats available will usually determine the style of habitat for being sampled in any respect sample stations. Locally modified sites, for instance small impoundments and bridge areas, really should be avoided unless data are needed to gauge their effects. Sampling nearby the mouths of tributaries entering large waterbodies should also be avoided simply because areas should have habitat more typical with the larger waterbody Karr et al. 1986. Rapid Bioassessment Protocols for Use in Streams and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish, Second Edition 3-7 For bioassessment activities in which the concern is non-chemical stressors, , the impact of habitat degradation or flow alteration, or cumulative impacts, a different procedure for station selection is employed. Physical habitat differences between sites might be substantial for 2 reasons: 1 one or possibly a set of web sites is more degraded physically than another, or 2 is unique for your stream class or region due on the essential natural structure presented by geological characteristics. Because of these situations, the greater critical the main siting process comes in the recognition on the habitat features which can be representative on the region or stream class. In basin-wide or watershed studies, sample locations must not be avoided as a result of habitat degradation or even physical features which can be well-represented within the stream class. 3.6 DATA MANAGEMENT AND ANALYSIS USEPA is creating a biological data management system associated with STORET, which gives a centralized system for storage of biological data and associated analytical tools for data analysis. The field survey file part of STORET provides a ways of storing, retrieving, and analyzing biosurvey data, all of which will process data for the distribution, abundance, and health of aquatic organisms, together with descriptions of the habitats. Data kept in STORET become part of your comprehensive database that might be used to be a reference, to refine analysis techniques or even define ecological requirements for aquatic populations. Data on the Rapid Bioassessment Protocols could be readily managed together with the STORET field survey file using header information presented within the field data forms Appendix A to recognize sampling stations. Habitat and physical characterization information can also be stored from the field survey file with organism abundance data. Parameters available inside field survey file can be employed to store some from the environmental characteristics associated while using sampling event, including physical characteristics, water quality, and habitat assessment. Physical/chemical parameters include stream depth, velocity, and substrate characteristics, together with many other parameters. STORET also allows storage of other pertinent station or sample information inside the comments section. Entering data to a computer system offers a substantial time savings. An additional benifit of computerization is analysis documentation, which is definitely an important component for any Quality Assurance/Quality Control QA/QC plan. An agency conducting rapid bioassessment programs can pick an existing system into their agency or utilize STORET system developed to be a national database system. Data collected within state bioassessment programs are generally entered, stored and analyzed in easily obtainable spreadsheet programs. This method of internet data management becomes cumbersome because the database grows in volume. An alternative to spreadsheet programs can be a. multiuser relational database management system ROMS. Most relational database software packages are designed with the Windows operating system and provides menu driven interfaces and ranges of toolbars that offer quick access to many people routine database tasks. Automated tools help users quickly create forms for data input and lookup, tables, reports, and sophisticated queries regarding the data. The USEPA is creating a multiuser relational database management system that could transfer sampling data to STORET. This relational database management method is EDAS Ecological Data Application System and allows anyone to input, compile, and analyze complex ecological data to produce assessments of ecosystem condition. EDAS includes tools to format sampling data so it can be loaded into STORET like a batch file. These batch files are formatted as flat ASCII text and may be loaded transferred electronically to STORET. This will eliminate the desire to key sample data into STORET. 3-8 Chapter 3: Elements ofBiomonitoring By using tables and queries as established in EDAS, a person can enter, manipulate, and print data. The metrics utilized in most bioassessments could be calculated with simple queries that have been created for your user. New queries might be created so additional metrics may be calculated in the click with the mouse each and every time data are updated or changed. If an operation around the data would be to complex for one on the many default functions next the function might be written in code, visual basic access and stored inside a module in order to use in any query. Repetitive steps is usually handled with macros. As the consumer develops the database other database elements like forms and reports is usually added. EDASv2.p-Relationships Edit 0ew aetotkHuhlpc loob Wmttm Help i I 1 I ! Rea Stations SfreamNama j Photo 1 Location Basin BasinID Order Catchment Area:, Physiographic Rsgfcx.; Ecoregton, y Type -. Stats. -,,, -. -.!, 1 sss f 1 i i sf F! e S f BenSomps Bensampro El RepNum StatlonID - v Grids ;;;- HabSamps HabSampID ttj StatlonID fj CollDate iterDate. Field Team JVJ ChsmSamps ChemSamplD. I AssembSamp!J StationID - CoIDate f Fill your social calendar while you possess a great time. 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